At the 34° Congresso Nazionale della Società Italiana di Farmacologia in Rimini, Pala-congressi, 14-17 Ottobre 2009, Marta Valenti and colleagues presented data on the ROLE OF PALMITOYLETHANOLAMIDE ON MODULATION OF THE MAST CELL-T CELL INTERACTIONS: AN IN VITRO STUDY.
They analyzed supernatants obtained from co-culturing (human mast cells and activated Jurkat T cells with different concentrations of PEA (nM range) or its vehicle, for released IL-8.
PEA was able to decrease the amount of released IL-8 (~ 45%) in a concentration-independent manner.
To analyse whether PEA was able to interact per se with mast cells or T cells activity, the authors performed experiments in which HMC-1 or activated Jurkat T cells were treated with PEA before carrying out co-cultures.
There was no differences in IL-8 release when supernatants from pre-treated HMC-1 co-cultured with activated Jurkat T cells were analyzed, but, surprisingly, a decrease by 75% was observed in supernatants obtained from pre-treated Jurkat T cells and HMC-1. The CB2 receptor was not involved in PEA effects as demonstrated by the inefficacy of SR144528 antagonist in reverting the IL-8 inhibition.
These results suggest that PEA can effectively modulate the mast cells-T cell interaction, corroborating its valuable anti-inflammatory efficacy.
Study was sponsored by Epitech